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dominant negative tcf4 dntcf4 constructs  (Addgene inc)
91
Addgene inc dominant negative tcf4 dntcf4 constructs
Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either <t>dnTCF4</t> or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.
Dominant Negative Tcf4 Dntcf4 Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dominant negative tcf4 dntcf4 constructs - by Bioz Stars, 2026-05
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lentiviral dominant negative tcf4 plasmid  (Addgene inc)
88
Addgene inc lentiviral dominant negative tcf4 plasmid
Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either <t>dnTCF4</t> or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.
Lentiviral Dominant Negative Tcf4 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lentiviral dominant negative tcf4 plasmid - by Bioz Stars, 2026-05
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dominant-negative tcf-4 (dn-tcf4)  (Shanghai GenePharma)
90
Shanghai GenePharma dominant-negative tcf-4 (dn-tcf4)
Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either <t>dnTCF4</t> or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.
Dominant Negative Tcf 4 (Dn Tcf4), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dominant-negative tcf-4 (dn-tcf4)/product/Shanghai GenePharma
Average 90 stars, based on 1 article reviews
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plasmid encoding human dominant-negative tcf4 (dntcf4) [ppgs dntcf-4(deltan41)]  (Addgene inc)
90
Addgene inc plasmid encoding human dominant-negative tcf4 (dntcf4) [ppgs dntcf-4(deltan41)]
Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either <t>dnTCF4</t> or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.
Plasmid Encoding Human Dominant Negative Tcf4 (Dntcf4) [Ppgs Dntcf 4(deltan41)], supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plasmid encoding human dominant-negative tcf4 (dntcf4) [ppgs dntcf-4(deltan41)]/product/Addgene inc
Average 90 stars, based on 1 article reviews
plasmid encoding human dominant-negative tcf4 (dntcf4) [ppgs dntcf-4(deltan41)] - by Bioz Stars, 2026-05
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dominant negative tcf4 dntcf4  (Addgene inc)
93
Addgene inc dominant negative tcf4 dntcf4
Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either <t>dnTCF4</t> or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.
Dominant Negative Tcf4 Dntcf4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dominant negative tcf4 dntcf4/product/Addgene inc
Average 93 stars, based on 1 article reviews
dominant negative tcf4 dntcf4 - by Bioz Stars, 2026-05
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pcdna3 delta n human tcf4 tcf4dn  (Addgene inc)
92
Addgene inc pcdna3 delta n human tcf4 tcf4dn
Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either <t>dnTCF4</t> or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.
Pcdna3 Delta N Human Tcf4 Tcf4dn, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcdna3 delta n human tcf4 tcf4dn/product/Addgene inc
Average 92 stars, based on 1 article reviews
pcdna3 delta n human tcf4 tcf4dn - by Bioz Stars, 2026-05
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dominant negative tcf4  (Addgene inc)
92
Addgene inc dominant negative tcf4
Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either <t>dnTCF4</t> or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.
Dominant Negative Tcf4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dominant negative tcf4/product/Addgene inc
Average 92 stars, based on 1 article reviews
dominant negative tcf4 - by Bioz Stars, 2026-05
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dominant-negative-tcf4 plasmid  (Millipore)
90
Millipore dominant-negative-tcf4 plasmid
Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either <t>dnTCF4</t> or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.
Dominant Negative Tcf4 Plasmid, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dominant-negative-tcf4 plasmid/product/Millipore
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dominant-negative-tcf4 plasmid - by Bioz Stars, 2026-05
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dominant-negative tcf4 plasmid ppgs dntcf-4(deltan41)  (Addgene inc)
90
Addgene inc dominant-negative tcf4 plasmid ppgs dntcf-4(deltan41)
Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either <t>dnTCF4</t> or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.
Dominant Negative Tcf4 Plasmid Ppgs Dntcf 4(deltan41), supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dominant-negative tcf4 plasmid ppgs dntcf-4(deltan41)/product/Addgene inc
Average 90 stars, based on 1 article reviews
dominant-negative tcf4 plasmid ppgs dntcf-4(deltan41) - by Bioz Stars, 2026-05
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Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either dnTCF4 or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.

Journal: Neoplasia (New York, N.Y.)

Article Title: Targeting Wnt/β-catenin signaling enhances the efficacy of anti-CD38 immunotherapy in multiple myeloma

doi: 10.1016/j.neo.2025.101242

Figure Lengend Snippet: Inhibition of Wnt signaling suppresses STAT3 signaling in MM. (A) Immunoblot analysis of p-STAT3 (phospho-STAT3) and t-STAT3 (total-STAT3) expression in HMCLs transduced with either dnTCF4 or empty vector (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001 using Student’s t-test. (B-C) Analysis of IL-6 (B), c-MYC and CCND1 (C) mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (24 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Immunoblot analysis of β-catenin, p-STAT3 and t-STAT3 protein expression in HMCLs transduced with non-targeting (NT) CRISPR, β-catenin sgRNA1 or sgRNA2 CRISPR (48 h after transduction). β-actin served as loading control. ImageJ was used for immunoblot quantitative analysis. The mean ± SD of three independent experiments in triplicate is shown. ns non-significant; *** p ≤ 0.001; **** p ≤ 0.0001 using one-way ANOVA with the Tukey post hoc test.

Article Snippet: The dominant negative TCF4 (dnTCF4) constructs were obtained from Addgene (24310); The STAT3-GFP constructs were obtained from Addgene (110495) [ ].

Techniques: Inhibition, Western Blot, Expressing, Transduction, Plasmid Preparation, Control, CRISPR

Inhibition of Wnt signaling upregulates CD38 expression in MM. (A) Analysis of CD38 mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (48 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. * p ≤ 0.05; *** p ≤ 0.001 using Student’s t-test. (B) Flow cytometry analysis of cell-surface expression of CD38 in control empty vector-transduced (EV control) and dnTCF4-transduced HMCLs (48 h after transduction). A representative plot of three independent experiments is shown. (C) Flow cytometry analysis of cell-surface expression of CD38 in control empty vector-transduced (EV control) and dnTCF4-transduced HMCLs (48 h after transduction), and mean fluorescence intensity (MFI) is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Analysis of CD38 mRNA expression in the HMCLs after ICG-001 (5 µM) treatment for 24 h by qPCR. The mean ± SD of three independent experiments in triplicate is shown. * p ≤ 0.05; ** p ≤ 0.01 using Student’s t-test. (E) Flow cytometry analysis of HMCLs cell-surface CD38 expression after ICG-001 (5 µM) treatment for 24 h.

Journal: Neoplasia (New York, N.Y.)

Article Title: Targeting Wnt/β-catenin signaling enhances the efficacy of anti-CD38 immunotherapy in multiple myeloma

doi: 10.1016/j.neo.2025.101242

Figure Lengend Snippet: Inhibition of Wnt signaling upregulates CD38 expression in MM. (A) Analysis of CD38 mRNA expression in the HMCLs transduced with dnTCF4 or empty vector (EV) by qPCR (48 h after transduction). The mean ± SD of three independent experiments in triplicate is shown. * p ≤ 0.05; *** p ≤ 0.001 using Student’s t-test. (B) Flow cytometry analysis of cell-surface expression of CD38 in control empty vector-transduced (EV control) and dnTCF4-transduced HMCLs (48 h after transduction). A representative plot of three independent experiments is shown. (C) Flow cytometry analysis of cell-surface expression of CD38 in control empty vector-transduced (EV control) and dnTCF4-transduced HMCLs (48 h after transduction), and mean fluorescence intensity (MFI) is shown. ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001 using Student’s t-test. (D) Analysis of CD38 mRNA expression in the HMCLs after ICG-001 (5 µM) treatment for 24 h by qPCR. The mean ± SD of three independent experiments in triplicate is shown. * p ≤ 0.05; ** p ≤ 0.01 using Student’s t-test. (E) Flow cytometry analysis of HMCLs cell-surface CD38 expression after ICG-001 (5 µM) treatment for 24 h.

Article Snippet: The dominant negative TCF4 (dnTCF4) constructs were obtained from Addgene (24310); The STAT3-GFP constructs were obtained from Addgene (110495) [ ].

Techniques: Inhibition, Expressing, Transduction, Plasmid Preparation, Flow Cytometry, Control, Fluorescence